Originally Posted by TimothyH

First step is to mark the tire and rim at the high spot with a piece of chalk or sharpie or something similar. Put a line from the tread, down the sidewall and across the rim right at the high spot.

Then take the tire off, rotate it 180° on the rim and reinstall.

If the high spot stays where it is then the problem is with the rim, rim tape or tube. If the high spot moves to the other side of the wheel then the tire is the problem.