Probably if you can find equipment accurate enough and can eliminate every other factor. (Most people would call this obsessive.)
Real life - I've patched many tubes many times and the only time I notice the patches is when I pull the tube out. My norm at tube replacement is 5 patches. Now, 5 patches does add some weight but not a lot. A small REMA patch kit weighs maybe an half an ounce. That (edit) half ounce includes 6 patches, one of which is large, a tube, a casing patch and sandpaper. The box itself weighs probably 1/'3 the total. If my guesses are close, the rest weighs 10g. So maybe 5 grams for 5 patches and glue used. On a 70 gram tire. ON a 1000g wheel. On a 7000g bike. Ridden by a 70,000g rider. I would expect the additional rolling resistance to play out about the same.
Last edited by 79pmooney; 03-19-20 at 01:01 PM.