Addiction LXXX
#5476
Klaatu..Verata..Necktie?
Join Date: May 2007
Location: SF Bay Area
Posts: 17,662
Bikes: Litespeed Ultimate, Ultegra; Canyon Endurace, 105; Battaglin MAX, Chorus; Bianchi 928 Veloce; Ritchey Road Logic, Dura Ace; Cannondale R500 RX100; Schwinn Circuit, Sante; Lotus Supreme, Dura Ace
Mentioned: 41 Post(s)
Tagged: 0 Thread(s)
Quoted: 10247 Post(s)
Liked 11,600 Times
in
5,946 Posts
Note also that RNA is much more labile than DNA. We always store RNA preps at -80, and ship on dry ice. DNA, we sometime ship ambient.
__________________
"Don't take life so serious-it ain't nohow permanent."
"Everybody's gotta be somewhere." - Eccles
"Don't take life so serious-it ain't nohow permanent."
"Everybody's gotta be somewhere." - Eccles
#5477
Join Date: Jun 2015
Location: Land of Pleasant Living
Posts: 12,155
Bikes: Shmikes
Mentioned: 58 Post(s)
Tagged: 0 Thread(s)
Quoted: 10104 Post(s)
Liked 5,784 Times
in
3,112 Posts
#5478
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5479
serious cyclist
Join Date: Apr 2009
Location: Austin
Posts: 21,147
Bikes: S1, R2, P2
Mentioned: 115 Post(s)
Tagged: 1 Thread(s)
Quoted: 9334 Post(s)
Liked 3,679 Times
in
2,026 Posts
#5480
Join Date: Jun 2015
Location: Land of Pleasant Living
Posts: 12,155
Bikes: Shmikes
Mentioned: 58 Post(s)
Tagged: 0 Thread(s)
Quoted: 10104 Post(s)
Liked 5,784 Times
in
3,112 Posts
Likes For MoAlpha:
#5481
Super Modest
Join Date: Jun 2002
Location: Central Illinois
Posts: 23,394
Bikes: Trek Emonda, Giant Propel, Colnago V3, Co-Motion Supremo, ICE VTX WC
Mentioned: 107 Post(s)
Tagged: 1 Thread(s)
Quoted: 10894 Post(s)
Liked 4,560 Times
in
2,090 Posts
Dang, wish that I would have ridden at least 3.1 more miles today.
__________________
Keep the chain tight!
#5482
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts
#5483
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5484
serious cyclist
Join Date: Apr 2009
Location: Austin
Posts: 21,147
Bikes: S1, R2, P2
Mentioned: 115 Post(s)
Tagged: 1 Thread(s)
Quoted: 9334 Post(s)
Liked 3,679 Times
in
2,026 Posts
#5485
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5486
Join Date: Jun 2015
Location: Land of Pleasant Living
Posts: 12,155
Bikes: Shmikes
Mentioned: 58 Post(s)
Tagged: 0 Thread(s)
Quoted: 10104 Post(s)
Liked 5,784 Times
in
3,112 Posts
#5487
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5488
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts
Dangit, I missed Gordon Lightfoot's 82nd birthday yesterday
#5489
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5490
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts
Did you know that I-19 has kilometre posts in addition to mile posts? #yourtaxdollarsatwork
#5491
Senior Member
Join Date: Sep 2005
Location: In the foothills of Los Angeles County
Posts: 25,107
Mentioned: 8 Post(s)
Tagged: 0 Thread(s)
Quoted: 8191 Post(s)
Liked 8,849 Times
in
4,396 Posts
I was climbing the closed road today when 3 e-bikes flew past me, just as some hikers were walking the other direction. One of the girls hiking asked me if she should give me a push. I guess she felt sorry for me not keeping up with the other bikes. I told her they were e-bikes and I was going to get one when I turn 70.
#5492
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5493
Klaatu..Verata..Necktie?
Join Date: May 2007
Location: SF Bay Area
Posts: 17,662
Bikes: Litespeed Ultimate, Ultegra; Canyon Endurace, 105; Battaglin MAX, Chorus; Bianchi 928 Veloce; Ritchey Road Logic, Dura Ace; Cannondale R500 RX100; Schwinn Circuit, Sante; Lotus Supreme, Dura Ace
Mentioned: 41 Post(s)
Tagged: 0 Thread(s)
Quoted: 10247 Post(s)
Liked 11,600 Times
in
5,946 Posts
True. Usually we add glycerol or DMSO to cryopreserve things because freezing cells, or even proteins in aqueous solutions without it will **** them up but good.
__________________
"Don't take life so serious-it ain't nohow permanent."
"Everybody's gotta be somewhere." - Eccles
"Don't take life so serious-it ain't nohow permanent."
"Everybody's gotta be somewhere." - Eccles
#5494
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5495
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts
#5496
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts
#5497
Should Be More Popular
Join Date: Dec 2007
Location: Malvern, PA (20 miles West of Philly)
Posts: 42,957
Bikes: 1986 Alpine (steel road bike), 2009 Ti Habenero, 2013 Specialized Roubaix
Mentioned: 560 Post(s)
Tagged: 2 Thread(s)
Quoted: 22513 Post(s)
Liked 8,843 Times
in
4,113 Posts
Likes For datlas:
#5498
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts
Princess says hi Jackie. (yes, princess has a throne.)
Likes For LesterOfPuppets:
#5499
VFL For Life
Join Date: Feb 2005
Location: Knoxville, TN
Posts: 51,049
Bikes: Velo Volmobile
Mentioned: 780 Post(s)
Tagged: 2 Thread(s)
Quoted: 28439 Post(s)
Liked 1,832 Times
in
1,300 Posts
#5500
cowboy, steel horse, etc
Join Date: Sep 2008
Location: The hot spot.
Posts: 44,801
Bikes: everywhere
Mentioned: 71 Post(s)
Tagged: 1 Thread(s)
Quoted: 12635 Post(s)
Liked 7,528 Times
in
3,989 Posts