Originally Posted by FBinNY

Note, if the cassette position varies enough to require adjustment of the limits, it's very likely that it will also require adjusting the RD trim to index properly.

Also note that while makers of index cassettes are very diligent about sprocket spacing on all the inner sprockets, and likewise the lever's index cam, they allow themselves greater margin of error for the high gear sprocket. The index system may allow an overshift, or slack cable condition with the assumption that the RD position will depend of the limit rather than the lever.